We make "YOUR FUTURE"

  【 Our purpose 】

1.Evalute the search for substances that respond (interact) to the targets based on
 the intellectual property posseessed by Caretis.
2.Utilization for the purpose of visualizing intermolecular interactions.
3.共Utilization for in vitro experiments to demonstrate natural frequency of organisms.

  ［ Ⅱ.Detection principle ］

A crystal oscillator is an electric element having a structure in which metal films are attached to
both side of a section obtained by cutting a crystal into an extremely thin plate, and when
an AC electric field is applied to the crystal section through both metal thin films, a certain
frequency is applied. It has been found that when resonance frequency decreases according to its
mass, so that it can be used as a trace balance. Such a meathod is QCM (Quartz-Crystal
Microbalance) is said to be.

  ［ Ⅰ.Equipment assembly example ］

Structure of crystal oscillator

Schematic diagram of frequency change measuring device in liquid phase

  ［ Ⅲ.Example of measurement target ］

①

②

③

①：Monomolecular membrane ―　Protein
②：DNA（ RNA ) ー DNA ( RNA )
           Protein(peptide)
           Snythetic compound
③：Protein(peptide) ― Protein(peptide)
             Microbal（bacteria, virus, etc.）
             Synthetic compound,　etc.

  ［ Ⅳ.Measurement results ］

Arrows indicate points of addition of binding substances with different concentrations.
Frequency decreases with interaction.

  ［ Ⅴ.Analysis method（dessociation constant:K_ｄ） ］  Method for analyzing the strength of interaction.

（Law of mass action）

K_d : dissociation constant
ｋ₁ : binding rate
ｋ₂ : dissociation rate
A : acceptor (bound to the coated surface)
B : binding substance
AB : complex
B₀ : total amount of B
M : molecular weight of B
V : reaction system volume
Δm : mass change per unit area of QCM sensor surface
Δm_max : maximum coupling amount of B
S : QCM sensor surface area

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